ASRB NET Agricultural Biotechnology Syllabus
Now candidates can download ASRB NET Agricultural Biotechnology syllabus from this website. Candidates need to go through ASRB NET syllabus in detail before starting preparing for the exam. Aspirant candidates always looking for ASRB NET Agricultural Biotechnology books & preparation materials with solutions. Our website helps students to crack to this ASRB NET exam.
The ASRB NET selection process consists of a single-stage that is the online Computer Based Test (CBT). ASRB NET exam pattern remains the same for all disciplines. Every candidate has to appear for only one paper that he/she has opted for. Exams for different disciplines will be conducted separately, candidates will be communicated regarding the exam through ASRB NET admit card.
The Test will comprise of one paper of 150 marks consisting of 150 objective type multiple choice questions (from the respective discipline opted by the candidate) to be solved in 2 hours duration.
Agricultural Biotechnology
Unit 1: Cell Structure and Function
Prokaryotic and eukaryotic cell architecture, Cell wall, plasma membrane, Structure and function of cell organelles: vacuoles, mitochondria, plastids, golgi apparatus, ER, peroxisomes, glyoxisomes. Cell division, regulation of cell cycle, Protein secretion and targeting, Cell division, growth, and differentiation.
Unit 2: Biomolecules and Metabolism
Structure and function of carbohydrates, lipids, proteins, and nucleic acids, Synthesis of carbohydrates, glycolysis, HMP, Citric acid cycle and metabolic regulation, Oxidative phosphorylation, and substrate-level phosphorylation, Vitamins, plant, and animal hormones. Functional molecules, antioxidants, nutrient precursors, HSPs, anti-viral compounds.
Unit 3: Enzymology
Enzymes, structure conformation, classification, assay, isolation, purification and characterization, catariysis specificity, mechanism of action, active site, regulation of enzyme activity, multienzyme complexes, immobilized enzymes and protein engineering, immobilized enzymes, and their application.
Unit 4: Molecular Genetics
Concept of the gene, Prokaryotes as a genetic system, Prokaryotic and eukaryotic chromosomes, methods of gene isolation and identification, Split genes, overlapping genes, and pseudogenes, Organization of prokaryotic and eukaryotic genes and genomes including opera, exon, intron, enhancer-promoter sequences, and other regulatory elements. Mutation – spontaneous, induced, and site-directed, recombination in bacteria, fungi, and viruses, transformation, transduction, conjugation, transposable elements, and transposition.
Unit 5: Gene Expression
Expression of genetic information, operon concept, Transcription – mechanism of transcription in prokaryotes and eukaryotes, transcription unit, regulatory sequences and enhancers, activators, repressors, co-activators, Co-repressors in prokaryotes and eukaryotes, inducible genes and promoters, Transcription factors post-transcriptional modification and protein transport, DNA-protein interaction, Genetic
code. Mechanism of translation and its control, post-translational modifications.
Unit 6: Molecular Biology Techniques
Isolation and purification of nucleic acids. Nucleic acids hybridization: Southern, northern, and western blotting hybridization. Immune response monoclonal and polyclonal antibodies and ELISA, DNA sequencing. Construction and screening of genomic and C-DNA libraries. Gel electrophoretic techniques. Polymerase chain reactor spectroscopy, rtPCR ultracentrifugation, chromatography, FISH, RIA, etc.
Unit 7: Gene Cloning
Restriction enzymes and their uses. Salient features and uses of most commonly used vectors i.e. plasmids, bacteriophages, phagmids, cosmids, BACs, PACs and YACs, binary vectors, expression vectors. Gene cloning and sub-cloning strategies, chromosome walking, genetic transformation, Basis of animal cloning. Biology. Risk assessment and IPR.
Unit 8: Molecular Biology 2
Ribosome structure and function. Protein biosynthesis in prokaryotes and ekaryotes. Post-translational modification. Gene regulation, RNA processing, and Post-transcriptional modifications. Bioprospecting, biofortification, gene pyramiding and gene fusion, nbozyme technology.
Unit 9: Plant Molecular Biology
Photoregulation and phytochrome regulation of nuclear and chloroplastic gene expression. Molecular mechanism of nitrogen fixation. Molecular biology of various stresses, viz. abiotic stresses like drought, salt, heavy metals, and temperature; and biotic stresses like bacterial, fungal, and viral diseases. Signal transduction and its molecular basis, the molecular mechanism of plant hormone action mitochondrial control of fertility, structure, organization, and regulation of nuclear gene concerning storage proteins and starch synthesis.
Unit 10: Tissue Culture
Basic techniques in cell culture and somatic cell genetics. Regulation of cell cycle and cell division.. Clonal propagation. Concept of cellular totipotency. Anther culture, somaclonal and gametoclonal variations. Hybrid embryo culture and embryo rescue, somatic hybridization and cybridization. Application of tissue culture in crop improvement. Secondary metabolite production. In vitro, mutagenesis, cryopreservation and plant tissue culture repository.
Unit 11: Plant Genetic Engineering
Isolation of genes of economic importance. Gene constructs for tissue-specific expression. Different methods of gene transfer to plants, viz. direct and vector-mediated. Molecular analysis of transformants. Potential applications of plant genetic engineering for crop improvement, i.e. insect-pest resistance (insect, viral, fungal, and bacterial disease resistance), abiotic stress resistance, herbicide resistance, storage
protein quality, increasing shelf-life, oil quality, Current status of transgenics, biosafety norms, and controlled field trials and release of transgenics (GMOs).
Unit 12: Molecular Markers and Genomics
DNA molecular markers: Principles, type, and applications; restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA sequences (RAPD), Simple sequence repeats (SSR), Single nucleotide polymorphism (SNP), Structural and functional genomics, gene mapping, genome mapping, gene tagging and comparative genomics and application of genomics.